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磷酸化氨基末端激酶1/2/3重組兔單克隆抗體
  • 產(chǎn)品貨號(hào):
    BN42133R
  • 中文名稱:
    磷酸化氨基末端激酶1/2/3重組兔單克隆抗體
  • 英文名稱:
    Rabbit anti-Phospho-JNK1/2/3(T183+T183+T221) Monoclonal antibody
  • 品牌:
    Biorigin
  • 貨號(hào)

    產(chǎn)品規(guī)格

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  • BN42133R-50ul

    50ul

    ¥2020.00

    交叉反應(yīng):Rat,Mouse,Human 推薦應(yīng)用:WB,IHC-P,IHC-F,ICC,Flow-Cyt

  • BN42133R-100ul

    100ul

    ¥3240.00

    交叉反應(yīng):Rat,Mouse,Human 推薦應(yīng)用:WB,IHC-P,IHC-F,ICC,Flow-Cyt

產(chǎn)品描述

英文名稱Phospho-JNK1/2/3(T183+T183+T221)
中文名稱磷酸化氨基末端激酶1/2/3重組兔單克隆抗體
別    名JNK1 + JNK2 + JNK3 (phospho T183 + T183 + T221); JNK1 (phospho T183); p-JNK1 (phospho T183); MAPK8 (phospho T183); JNK1 + JNK2 (phospho Thr183 + Thr183); JNK1 + 2 (phospho Thr183+Thr183); p-JNK; c Jun N terminal kinase 1; C-JUN kinase 1; EC 2.7.11.24; JAK 1A; JAK1A; JNK 1; JNK 46; JNK; JNK1A2; JNK21B1/2; MAP kinase 8; MAPK 8; MAPK8; Mitogen activated protein kinase 8; p54 gamma; PRKM 8; PRKM8; Protein kinase JNK1; Protein kinase, mitogen-activated, 8; SAPK 1; SAPK gamma; SAPK1; Stress activated protein kinase JNK1; Stress-activated protein kinase JNK1; Tyrosine protein kinase JAK1; AI849689; MK08_HUMAN; MK09_HUMAN; MK10_HUMAN.  
研究領(lǐng)域腫瘤  信號(hào)轉(zhuǎn)導(dǎo)  轉(zhuǎn)錄調(diào)節(jié)因子  
抗體來(lái)源Rabbit
克隆類型Monoclonal
克 隆 號(hào)6C10
交叉反應(yīng)Human, Mouse, Rat, 
產(chǎn)品應(yīng)用WB=1:1000-2000 IP=1:20-50 IHC-P=1:50-200 IHC-F=1:50-200 Flow-Cyt=1:100 ICC=1:50 (石蠟切片需做抗原修復(fù))
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量42kDa
細(xì)胞定位細(xì)胞核 細(xì)胞漿 
性    狀Lyophilized or Liquid
濃    度1mg/ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human JNK1/JNK2/JNK3 around the phosphorylation site of T183/T183/T221:MM(p-T)PY  
亞    型IgG
純化方法affinity purified by Protein A
儲(chǔ) 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
產(chǎn)品介紹The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Five alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jun 2013]

Function:
Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation. Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy. Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone. Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH.
JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.

Subcellular Location:
Cytoplasm. Nucleus.

Post-translational modifications:
Dually phosphorylated on Thr-183 and Tyr-185 by MAP2K7 and MAP2K4, which activates the enzyme. Phosphorylated by TAOK2.

Similarity:
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain.

SWISS:
P45983

Gene ID:
5599

Database links:

Entrez Gene: 5599 Human

Entrez Gene: 5601 Human

Entrez Gene: 5602 Human

Entrez Gene: 26414 Mouse

Entrez Gene: 26419 Mouse

Entrez Gene: 26420 Mouse

Entrez Gene: 116554 Rat

Entrez Gene: 25272 Rat

Entrez Gene: 50658 Rat

Omim: 601158 Human

Omim: 602896 Human

Omim: 602897 Human

SwissProt: P45983 Human

SwissProt: P45984 Human

SwissProt: P53779 Human

SwissProt: Q61831 Mouse

SwissProt: Q91Y86 Mouse

SwissProt: Q9WTU6 Mouse

SwissProt: P49185 Rat

SwissProt: P49186 Rat

SwissProt: P49187 Rat

Unigene: 138211 Human

Unigene: 522924 Human

Unigene: 21495 Mouse

Unigene: 4090 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.