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細胞角蛋白8單克隆抗體
  • 產(chǎn)品貨號：
    BN41989M
  • 中文名稱：
    細胞角蛋白8單克隆抗體
  • 英文名稱：
    Mouse anti-Cytokeratin 8 Monoclonal antibody
  • 品牌：
    Biorigin

  • 貨號

    產(chǎn)品規(guī)格

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  • BN41989M-50ul

    50ul

    ￥1486.00

    交叉反應：Human,Mouse,Rat 推薦應用：IHC-P,IHC-F,IF,Flow-Cyt

  • BN41989M-100ul

    100ul

    ￥2360.00

    交叉反應：Human,Mouse,Rat 推薦應用：IHC-P,IHC-F,IF,Flow-Cyt

產(chǎn)品描述

英文名稱Cytokeratin 8
中文名稱細胞角蛋白8單克隆抗體
別    名card2; Cardiac autoantigen 2 120kD; CK 8; CK8; CK-8; ck8; Cyk 8; cyk8; CYKER; Cytokeratin endo A; Cytokeratin-8; Cytokeratin8; DreK8; EndoA; k0; CYK8; k2c8; K2C8_HUMAN; k8; Keratin 8; Keratin type ii cytoskeletal 8; Keratin, type II cytoskeletal 8; Keratin-8; Keratin8; KO; Krt 2.8; Krt 8; krt8; KRT-8; MGC118110; MGC174782; MGC53564; MGC85764; sb:cb186; Type-II keratin Kb8.  
研究領(lǐng)域腫瘤  細胞生物  信號轉(zhuǎn)導  
抗體來源Mouse
克隆類型Monoclonal
克 隆 號10A8
交叉反應Human, Mouse, Rat, 
產(chǎn)品應用IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:200-800 （石蠟切片需做抗原修復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量53kDa
細胞定位細胞核 細胞漿 細胞膜 
性    狀Liquid
濃    度1mg/ml
免 疫 原Recombinant human CK8: 
亞    型IgG
純化方法affinity purified by Protein G
儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
產(chǎn)品介紹This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012].

Function:
Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

Subunit:
Heterotetramer of two type I and two type II keratins. KRT8 associates with KRT18. Associates with KRT20. Interacts with HCV core protein and PNN. When associated with KRT19, interacts with DMD. Interacts with TCHP. Interacts with APEX1.

Subcellular Location:
Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix.

Tissue Specificity:
Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.

Post-translational modifications:
Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.

DISEASE:
Defects in KRT8 are a cause of cirrhosis (CIRRH) [MIM:215600].

Similarity:
Belongs to the intermediate filament family.

SWISS:
P05787

Gene ID:
3856

Database links:

Entrez Gene: 3856 Human

Entrez Gene: 16691 Mouse

Entrez Gene: 25626 Rat

Omim: 148060 Human

SwissProt: P05787 Human

SwissProt: P11679 Mouse

SwissProt: Q10758 Rat

Unigene: 533782 Human

Unigene: 708445 Human

Unigene: 358618 Mouse

Unigene: 11083 Rat 



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

結(jié)構(gòu)蛋白（Structural Proteins）
上皮細胞胞質(zhì)中的骨架蛋白除微絲（肌動蛋白）、微管蛋白外，主要是細胞角蛋白（cytokeratins，CK）。CK8也是上皮細胞的特征性標記物：CK8存在于某些正常腺上皮及其腫瘤，包括許多導管上皮和腺上皮，如結(jié)腸、胃、小腸、氣管的上皮和尿路上皮。CK8主要用于腺癌和導管癌的診斷，鱗狀細胞癌一般不表達CK8。