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基質(zhì)金屬蛋白酶2抗體
  • 產(chǎn)品貨號(hào)：
    BN41907R
  • 中文名稱：
    基質(zhì)金屬蛋白酶2抗體
  • 英文名稱：
    Rabbit anti-MMP-2 Polyclonal antibody
  • 品牌：
    Biorigin

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  • BN41907R-50ul

    50ul

    ￥1486.00

    交叉反應(yīng)：Human,Mouse,Rat,Rabbit(predicted:Pig,Cow,Horse,Sheep) 推薦應(yīng)用：WB,IHC-P,IHC-F,ICC,IF,Flow-Cyt,ELISA

  • BN41907R-100ul

    100ul

    ￥2360.00

    交叉反應(yīng)：Human,Mouse,Rat,Rabbit(predicted:Pig,Cow,Horse,Sheep) 推薦應(yīng)用：WB,IHC-P,IHC-F,ICC,IF,Flow-Cyt,ELISA

  • BN41907R-200ul

    200ul

    ￥3490.00

    交叉反應(yīng)：Human,Mouse,Rat,Rabbit(predicted:Pig,Cow,Horse,Sheep) 推薦應(yīng)用：WB,IHC-P,IHC-F,ICC,IF,Flow-Cyt,ELISA

產(chǎn)品描述

英文名稱MMP-2
中文名稱基質(zhì)金屬蛋白酶2抗體
別    名MMP2; MMP 2; 72 kDa gelatinase; 72kD type IV collagenase; CLG 4; CLG 4A; CLG4; CLG4A; Collagenase Type 4 alpha; Collagenase type IV A; Gelatinase A; Gelatinase alpha; Gelatinase neutrophil; Matrix metallopeptidase 2 gelatinase A 72kDa gelatinase 72kDa type IV collagenase; Matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase); Matrix Metalloproteinase 2; Matrix metalloproteinase II; MMP 2; MMP II; MONA; Neutrophil gelatinase; TBE 1.  



研究領(lǐng)域腫瘤  免疫學(xué)  神經(jīng)生物學(xué)  合成與降解  
抗體來(lái)源Rabbit
克隆類型Polyclonal
交叉反應(yīng)Human, Mouse, Rat, Rabbit,  (predicted: Pig, Cow, Horse, Sheep, )
產(chǎn)品應(yīng)用WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test ICC=1:100 IF=1:100-500 （石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量62kDa
細(xì)胞定位細(xì)胞核 細(xì)胞漿 細(xì)胞膜 分泌型蛋白 
性    狀Liquid
濃    度1mg/ml
免 疫 原KLH conjugated synthetic peptide derived from human MMP-2:401-500/662 
亞    型IgG
純化方法affinity purified by Protein A
儲(chǔ) 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
產(chǎn)品介紹This gene is a member of the matrix metalloproteinase (MMP) gene family, that are zinc-dependent enzymes capable of cleaving components of the extracellular matrix and molecules involved in signal transduction. The protein encoded by this gene is a gelatinase A, type IV collagenase, that contains three fibronectin type II repeats in its catalytic site that allow binding of denatured type IV and V collagen and elastin. Unlike most MMP family members, activation of this protein can occur on the cell membrane. This enzyme can be activated extracellularly by proteases, or, intracellulary by its S-glutathiolation with no requirement for proteolytical removal of the pro-domain. This protein is thought to be involved in multiple pathways including roles in the nervous system, endometrial menstrual breakdown, regulation of vascularization, and metastasis. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]

Function:
Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues in association with MMP14.
PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels.
Isoform 2: Mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro-inflammatory NF-kappaB, NFAT and IRF transcriptional pathways.

Subunit:
Interacts (via the C-terminal hemopexin-like domains-containing region) with the integrin alpha-V/beta-3; the interaction promotes vascular invasion in angiogenic vessels and melamoma cells. Interacts (via the C-terminal PEX domain) with TIMP2 (via the C-terminal); the interaction inhibits the degradation activity. Interacts with GSK3B.

Subcellular Location:
Isoform 1: Secreted, extracellular space, extracellular matrix. Membrane. Nucleus. Note=Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes.
Isoform 2: Cytoplasm. Mitochondrion.

Tissue Specificity:
Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate.

Post-translational modifications:
Phosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro.
The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3.

DISEASE:
Multicentric osteolysis, nodulosis, and arthropathy (MONA) [MIM:259600]: An autosomal recessive syndrome characterized by severe multicentric osteolysis with predominant involvement of the hands and feet. Additional features include coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. {ECO:0000269|PubMed:11431697, disease is caused by mutations affecting the gene represented in this entry.

Similarity:
Belongs to the peptidase M10A family. {ECO:0000305}.
Contains 3 fibronectin type-II domains.
Contains 4 hemopexin repeats.

SWISS:
P08253

Gene ID:
4313

Database links:

Entrez Gene: 4313 Human

Entrez Gene: 17390 Mouse

Entrez Gene: 81686 Rat

Omim: 120360 Human

SwissProt: P08253 Human

SwissProt: P33434 Mouse

SwissProt: P33436 Rat

Unigene: 513617 Human

Unigene: 29564 Mouse

Unigene: 6422 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

基質(zhì)金屬蛋白酶(matrix metalloproteinases, MMPs)MMP2是一族依賴鋅離子而降解各種細(xì)胞外基質(zhì)的蛋白酶，亦稱IV型膠原酶或稱明膠酶A，其主要功能為降解IV型膠原，因而它在腫瘤細(xì)胞突破基底膜屏障和浸潤(rùn)轉(zhuǎn)移中起重要作用。目前主要用于各種惡性腫瘤(如乳腺癌、胃腸道癌、卵巢癌、膀胱癌等)中的基底膜檢測(cè)與轉(zhuǎn)移浸潤(rùn)的研究。

































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