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多腺苷二磷酸多聚酶抗體(N端)
  • 產(chǎn)品貨號：
    BN41785R
  • 中文名稱：
    多腺苷二磷酸多聚酶抗體(N端)
  • 英文名稱：
    Rabbit anti-PARP Polyclonal antibody
  • 品牌：
    Biorigin

  • 貨號

    產(chǎn)品規(guī)格

    售價

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  • BN41785R-50ul

    50ul

    ￥1486.00

    交叉反應(yīng)：Human,Mouse(predicted:Rat,Dog,Cow) 推薦應(yīng)用：WB,IHC-P,IHC-F,IF,Flow-Cyt,ELISA

  • BN41785R-100ul

    100ul

    ￥2360.00

    交叉反應(yīng)：Human,Mouse(predicted:Rat,Dog,Cow) 推薦應(yīng)用：WB,IHC-P,IHC-F,IF,Flow-Cyt,ELISA

  • BN41785R-200ul

    200ul

    ￥3490.00

    交叉反應(yīng)：Human,Mouse(predicted:Rat,Dog,Cow) 推薦應(yīng)用：WB,IHC-P,IHC-F,IF,Flow-Cyt,ELISA

產(chǎn)品描述

英文名稱PARP
中文名稱多腺苷二磷酸多聚酶抗體(N端)
別    名ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase); ADP ribosyltransferase NAD+; ADPRT 1; ADPRT; ADPRT1; msPARP; NAD(+) ADP ribosyltransferase 1; pADPRT 1; pADPRT1; PARP 1; PARP1; Poly (ADP ribose) polymerase 1; poly (ADP ribose) polymerase family, member 1; Poly adenosine diphosphate ADP ribose polymerase; Poly ADP ribose polymerase 1; Poly ADP ribose polymerase family member 1; Poly ADP ribose synthetase 1; poly(ADP ribose) synthetase; poly(ADP ribosyl)transferase; Poly[ADP ribose] synthetase 1; PPOL; sPARP 1; sPARP1; PARP1_HUMAN. 





研究領(lǐng)域染色質(zhì)和核信號  細胞凋亡  
抗體來源Rabbit
克隆類型Polyclonal
交叉反應(yīng)Human, Mouse,  (predicted: Rat, Dog, Cow, )
產(chǎn)品應(yīng)用WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=0.2μg/Test IF=1:100-500 （石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量111kDa
細胞定位細胞核 
性    狀Liquid
濃    度1mg/ml
免 疫 原KLH conjugated synthetic peptide derived from human PARP:201-300/1014 
亞    型IgG
純化方法affinity purified by Protein A
儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
產(chǎn)品介紹This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008].

Function:
Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production.

Subunit:
Component of a base excision repair (BER) complex, containing at least XRCC1, PARP2, POLB and LRIG3. Homo- and heterodimer with PARP2. Interacts with PARP3, APTX and SRY. The SWAP complex consists of NPM1, NCL, PARP1 and SWAP70. Interacts with TIAM2 and ZNF423 (By similarity). Interacts (when poly-ADP-ribosylated) with CHD1L. Interacts with the DNA polymerase alpha catalytic subunit POLA1; this interaction functions as part of the control of replication fork progression. Interacts with EEF1A1, RNF4 and TXK.

Subcellular Location:
Mitochondrion outer membrane; Single-pass membrane protein.
Nucleus membrane; Single-pass membrane protein.
Endoplasmic reticulum membrane; Single-pass membrane protein.
Nucleus.

Post-translational modifications:
Phosphorylated by PRKDC and TXK. Phosphorylated upon DNA damage, probably by ATM or ATR.
Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
S-nitrosylated, leading to inhibit transcription regulation activity.

Similarity:
Contains 1 BRCT domain.
Contains 1 PARP alpha-helical domain.
Contains 1 PARP catalytic domain.
Contains 2 PARP-type zinc fingers.

SWISS:
P09874

Gene ID:
142

Database links:

Entrez Gene: 142 Human

Entrez Gene: 11545 Mouse

Entrez Gene: 25591 Rat

Omim: 173870 Human

SwissProt: P09874 Human

SwissProt: P11103 Mouse

SwissProt: P27008 Rat

Unigene: 177766 Human

Unigene: 277779 Mouse

Unigene: 11327 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

PARP(poly ADP-ribose polymerase)是DNA修復(fù)酶。
PARP是細胞凋亡核心成員半胱胺酸蛋白酶(caspase)的切割底物。因此,它在DNA損傷修復(fù)與細胞凋亡中發(fā)揮著重要作用。Anti-PARP p85 是特意的PARPp85片段的特異抗體，由caspase剪切116kDa完整分子而得到的。
PARP是存在于多數(shù)真核細胞中的一個多功能蛋白質(zhì)翻譯后修飾酶。它通過識別結(jié)構(gòu)損傷的DNA片段而被激活，對聚腺苷二磷酸核糖聚合酶PARP被認為是DNA損傷的感受器。它還能對許多核蛋白進行聚腺苷二磷酸核糖基化。因此，在DNA損傷修復(fù)與細胞凋亡中發(fā)揮著重要作用，端錨聚合酶在癌細胞端粒結(jié)構(gòu)的調(diào)控機制中有重要作用。