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上皮鈣粘附分子抗體
  • 產(chǎn)品貨號(hào)：
    BN41725R
  • 中文名稱：
    上皮鈣粘附分子抗體
  • 英文名稱：
    Rabbit anti-E cadherin Polyclonal antibody
  • 品牌：
    Biorigin

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  • BN41725R-50ul

    50ul

    ￥1486.00

    交叉反應(yīng)：Human(predicted:Mouse,Rat,Chicken,Dog,Pig,Cow,Horse) 推薦應(yīng)用：WB,IHC-P,IHC-F,ICC,IF,Flow-Cyt,ELISA

  • BN41725R-100ul

    100ul

    ￥2360.00

    交叉反應(yīng)：Human(predicted:Mouse,Rat,Chicken,Dog,Pig,Cow,Horse) 推薦應(yīng)用：WB,IHC-P,IHC-F,ICC,IF,Flow-Cyt,ELISA

  • BN41725R-200ul

    200ul

    ￥3490.00

    交叉反應(yīng)：Human(predicted:Mouse,Rat,Chicken,Dog,Pig,Cow,Horse) 推薦應(yīng)用：WB,IHC-P,IHC-F,ICC,IF,Flow-Cyt,ELISA

產(chǎn)品描述

英文名稱E cadherin
中文名稱上皮鈣粘附分子抗體
別    名E-cadherin; anion exchanger protein 3; Arc 1; Cadherin 1; cadherin 1 type 1 E-cadherin; Cadherin1; CAM 120/80; CD 234; CD324; CD324 antigen; CDH1; CDHE; ECAD; Epithelial cadherin; epithelial calcium dependant adhesion protein; LCAM; Liver cell adhesion molecule; UVO; Uvomorulin; CADH1_HUMAN.  



研究領(lǐng)域腫瘤  細(xì)胞生物  免疫學(xué)  細(xì)胞粘附分子  細(xì)胞表面分子  上皮細(xì)胞  
抗體來源Rabbit
克隆類型Polyclonal
交叉反應(yīng)Human,  (predicted: Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, )
產(chǎn)品應(yīng)用WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test ICC=1:100 IF=1:100-500 （石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量90/97kDa
細(xì)胞定位細(xì)胞膜 
性    狀Liquid
濃    度1mg/ml
免 疫 原KLH conjugated synthetic peptide derived from human E-cadherin:841-882/882 <Cytoplasmic>
亞    型IgG
純化方法affinity purified by Protein A
儲(chǔ) 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
產(chǎn)品介紹This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16. [provided by RefSeq, Nov 2015]

Function:
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.
E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.

Subunit:
Homodimer.

Subcellular Location:
Cell junction. Cell membrane; Single-pass type I membrane protein.

Tissue Specificity:
Non-neural epithelial tissues.

Post-translational modifications:
During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3 to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disaaaembly of adherens junctions.

DISEASE:
Defects in CDH1 are involved in dysfunction of the cell-cell adhesion system, triggering cancer invasion (gastric, breast, ovary, endometrium and thyroid) and metastasis.
Defects in CDH1 are a cause of gastric cancer [MIM:137215]; also known as hereditary familial diffuse gastric cancer (HDGC).
Defects in CDH1 are a cause of susceptibility to endometrial cancer [MIM:608089].
Defects in CDH1 are associated with ovarian cancer [MIM:167000]. Ovarian cancer is the leading cause of death from gynecologic malignancy. It is characterized by advanced presentation with loco-regional dissemination in the peritoneal cavity and the rare incidence of visceral metastases. These typical features relate to the biology of the disease, which is a principal determinant of outcome.

Similarity:
Contains 5 cadherin domains.

SWISS:
P12830

Gene ID:
999

Database links:

Entrez Gene: 999 Human

Entrez Gene: 12550 Mouse

Entrez Gene: 83502 Rat

Omim: 192090 Human

SwissProt: P12830 Human

SwissProt: P09803 Mouse

SwissProt: Q9R0T4 Rat

Unigene: 461086 Human

Unigene: 35605 Mouse

Unigene: 1303 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

細(xì)胞粘附蛋白（Call Adhesion Protein）
E-chaherin是研究較多的同質(zhì)粘附分子。E-cadherin的表達(dá)與惡性腫瘤的分化程度、侵襲力、轉(zhuǎn)移負(fù)相關(guān)與預(yù)后正相關(guān)，E-cadherin的低表達(dá)和不穩(wěn)定表達(dá)可促進(jìn)轉(zhuǎn)移的發(fā)生。近年來已成為腫瘤細(xì)胞侵襲和轉(zhuǎn)移研究的熱點(diǎn)之一。