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膽固醇調(diào)節(jié)元件結(jié)合蛋白1抗體
  • 產(chǎn)品貨號(hào)：
    BN41705R
  • 中文名稱：
    膽固醇調(diào)節(jié)元件結(jié)合蛋白1抗體
  • 英文名稱：
    Rabbit anti-SREBP1 Polyclonal antibody
  • 品牌：
    Biorigin

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  • BN41705R-50ul

    50ul

    ￥1486.00

    交叉反應(yīng)：Human,Mouse,Rat(predicted:Chicken,Sheep) 推薦應(yīng)用：WB,IHC-P,IHC-F,IF,ELISA

  • BN41705R-100ul

    100ul

    ￥2360.00

    交叉反應(yīng)：Human,Mouse,Rat(predicted:Chicken,Sheep) 推薦應(yīng)用：WB,IHC-P,IHC-F,IF,ELISA

  • BN41705R-200ul

    200ul

    ￥3490.00

    交叉反應(yīng)：Human,Mouse,Rat(predicted:Chicken,Sheep) 推薦應(yīng)用：WB,IHC-P,IHC-F,IF,ELISA

產(chǎn)品描述

英文名稱SREBP1
中文名稱膽固醇調(diào)節(jié)元件結(jié)合蛋白1抗體
別    名Sterol regulatory element binding protein 1; ADD 1; BHLHD1; D630008H06; SREBF 1; SREBP-1; SREBF1; SREBP 1; SREBP1; SREBP 1c; Sterol regulatory element binding protein 1; Sterol Regulatory Element Binding Transcription Factor 1 / Protein 1; Sterol regulatory element binding transcription factor 1; SRBP1_HUMAN; Sterol regulatory element-binding protein 1; SREBP-1; Class D basic helix-loop-helix protein 1; bHLHd1; Sterol regulatory element-binding transcription factor 1; Processed sterol regulatory element-binding protein 1.  




研究領(lǐng)域心血管  轉(zhuǎn)錄調(diào)節(jié)因子  合成與降解  
抗體來(lái)源Rabbit
克隆類(lèi)型Polyclonal
交叉反應(yīng)Human, Mouse, Rat,  (predicted: Chicken, Sheep, )
產(chǎn)品應(yīng)用WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量54/126kDa
細(xì)胞定位細(xì)胞核 細(xì)胞漿 
性    狀Liquid
濃    度1mg/ml
免 疫 原KLH conjugated synthetic peptide derived from human SREBP-1:301-450/1147 
亞    型IgG
純化方法affinity purified by Protein A
儲(chǔ) 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
產(chǎn)品介紹This gene encodes a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. The protein is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Function:
Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway. Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3').

Subunit:
Forms a tight complex with SCAP in the ER membrane. Efficient DNA binding of the soluble transcription factor fragment requires dimerization with another bHLH protein. Interacts with LMNA.

Subcellular Location:
Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Cytoplasmic vesicle, COPII-coated vesicle membrane; Multi-pass membrane protein. Note=Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.
Processed sterol regulatory element-binding protein 1: Nucleus.
Isoform SREBP-1aDelta: Nucleus.
Isoform SREBP-1cDelta: Nucleus.

Tissue Specificity:
Expressed in a wide variety of tissues, most abundant in liver and adrenal gland. In fetal tissues lung and liver shows highest expression. Isoform SREBP-1C predominates in liver, adrenal gland and ovary, whereas isoform SREBP-1A predominates in hepatoma cell lines. Isoform SREBP-1A and isoform SREBP-1C are found in kidney, brain, white fat, and muscle.

Post-translational modifications:
At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7.
Phosphorylated by AMPK, leading to suppress protein processing and nuclear translocation, and repress target gene expression. Phosphorylation at Ser-402 by SIK1 represses activity possibly by inhibiting DNA-binding.

Similarity:
Belongs to the SREBP family.
Contains 1 bHLH (basic helix-loop-helix) domain.

SWISS:
P36956

Gene ID:
6720

Database links:

Entrez Gene: 6720 Human

Entrez Gene: 20787 Mouse

Entrez Gene: 78968 Rat

Omim: 184756 Human

SwissProt: P36956 Human

SwissProt: Q9WTN3 Mouse

SwissProt: P56720 Rat

Unigene: 592123 Human

Unigene: 278701 Mouse

Unigene: 296366 Mouse

Unigene: 198857 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

轉(zhuǎn)錄調(diào)節(jié)因子（Transcriptin Regulators）
膽固醇調(diào)節(jié)元件結(jié)合蛋白1(SREBP1)是膜結(jié)合的轉(zhuǎn)錄因子,參與脂質(zhì)自穩(wěn)態(tài)的許多作用(膽固醇、脂肪酸、甘油三酯和磷酯的合成/攝取).SREBP1在內(nèi)質(zhì)網(wǎng)上合成,需要被轉(zhuǎn)運(yùn)到高爾基體并經(jīng)過(guò)一系列蛋白水解后才能到核內(nèi)發(fā)揮作用。
未成熟時(shí)在胞漿表達(dá)，水解后的成熟片段在胞核表達(dá)。