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跨膜受體蛋白Notch-1抗體
  • 產(chǎn)品貨號：
    BN41689R
  • 中文名稱：
    跨膜受體蛋白Notch-1抗體
  • 英文名稱：
    Rabbit anti-Notch1 Polyclonal antibody
  • 品牌：
    Biorigin

  • 貨號

    產(chǎn)品規(guī)格

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  • BN41689R-50ul

    50ul

    ￥1486.00

    交叉反應：Human,Mouse,Rat 推薦應用：WB,IHC-P,IHC-F,IF,Flow-Cyt,ELISA

  • BN41689R-100ul

    100ul

    ￥2360.00

    交叉反應：Human,Mouse,Rat 推薦應用：WB,IHC-P,IHC-F,IF,Flow-Cyt,ELISA

  • BN41689R-200ul

    200ul

    ￥3490.00

    交叉反應：Human,Mouse,Rat 推薦應用：WB,IHC-P,IHC-F,IF,Flow-Cyt,ELISA

產(chǎn)品描述

英文名稱Notch1
中文名稱跨膜受體蛋白Notch-1抗體
別    名Notch 1 extracellular truncation; Notch 1 intracellular domain; hN1; Lin-12; LIN12; MIS6; Motch A; mT14; Neurogenic locus notch homolog protein 1; Neurogenic locus notch protein homolog; NICD; Notch1 intracellular domain; NOTC1_HUMAN; Notch 1; NOTCH; Notch gene homolog 1 (Drosophila); Notch homolog 1 translocation associated (Drosophila); Notch homolog 1, translocation-associated (Drosophila); NOTCH, Drosophila, homolog of, 1; notch1; p300; TAN 1; TAN1; TAN1; Translocation Associated Notch Homolog; Translocation Associated Notch Homolog; Translocation associated notch protein TAN 1; Translocation-associated notch protein TAN-1.  





研究領域細胞生物  發(fā)育生物學  神經(jīng)生物學  信號轉導  干細胞  表觀遺傳學  
抗體來源Rabbit
克隆類型Polyclonal
交叉反應Human, Mouse, Rat, 
產(chǎn)品應用WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （石蠟切片需做抗原修復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量86/89/271kDa
細胞定位細胞核 細胞膜 
性    狀Liquid
濃    度1mg/ml
免 疫 原KLH conjugated synthetic peptide derived from human C-terminal sequence of Notch 1 extracellular truncation and Notch 1 intracellular domain :2101-2300/2555 
亞    型IgG
純化方法affinity purified by Protein A
儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
產(chǎn)品介紹This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play multiple roles during development. [provided by RefSeq, Jul 2008].

Function:
Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia.

Subunit:
Heterodimer of a C-terminal fragment N(TM) and an N-terminal fragment N(EC) which are probably linked by disulfide bonds. Interacts with DNER, DTX1, DTX2 and RBPJ/RBPSUH. Also interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH1. The activated membrane-bound form interacts with AAK1 which promotes NOTCH1 stabilization. Forms a trimeric complex with FBXW7 and SGK1. Interacts with HIF1AN. HIF1AN negatively regulates the function of notch intracellular domain (NICD), accelerating myogenic differentiation.

Subcellular Location:
Cell membrane and Nucleus. Following proteolytical processing NICD is translocated to the nucleus.

Tissue Specificity:
In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues.

Post-translational modifications:
Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.
Phosphorylated.
O-glycosylated on the EGF-like domains. Contains both O-linked fucose and O-linked glucose.
Ubiquitinated; undergoes 'Lys-29'-linked polyubiquitination catalyzed by ITCH.

DISEASE:
Defects in NOTCH1 are a cause of bicuspid aortic valve (BAV) [MIM:109730]. A common defect in the aortic valve in which two rather than three leaflets are present. It is often associated with aortic valve calcification and insufficiency. In extreme cases, the blood flow may be so restricted that the left ventricle fails to grow, resulting in hypoplastic left heart syndrome.

Similarity:
Belongs to the NOTCH family.
Contains 5 ANK repeats.
Contains 36 EGF-like domains.
Contains 3 LNR (Lin/Notch) repeats.

SWISS:
P46531

Gene ID:
4851

Database links:

Entrez Gene: 4851 Human

Entrez Gene: 18128 Mouse

Entrez Gene: 25496 Rat

Omim: 190198 Human

SwissProt: P46531 Human

SwissProt: Q01705 Mouse

SwissProt: Q07008 Rat

Unigene: 495473 Human

Unigene: 290610 Mouse

Unigene: 25046 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

Notch 1蛋白是一個進化上保守的跨膜受體家族，廣泛分布和表達在多個物種之中。研究發(fā)現(xiàn)Notchl作為一條信號轉導途徑，不僅對正常組織、細胞的分化、發(fā)育起重要作用，而且和一些腫瘤的發(fā)生和生長相關，有學者發(fā)現(xiàn)Notchl在許多實體瘤中異常表達，如：如宮頸癌、子宮內(nèi)膜癌、腎癌、肺癌、乳腺癌、神經(jīng)母細胞瘤等。因此Notchl作為一種預防和治療腫瘤的新途徑越來越受到人們的重視。