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巢蛋白/神經上皮干細胞蛋白抗體
  • 產品貨號：
    BN41309R
  • 中文名稱：
    巢蛋白/神經上皮干細胞蛋白抗體
  • 英文名稱：
    Rabbit anti-Nestin Polyclonal antibody
  • 品牌：
    Biorigin

  • 貨號

    產品規(guī)格

    售價

    備注

  • BN41309R-100ul

    100ul

    ￥2360.00

    交叉反應：Human(predicted:Cow) 推薦應用：IHC-P,IHC-F,ICC,IF,Flow-Cyt,ELISA

  • BN41309R-200ul

    200ul

    ￥3490.00

    交叉反應：Human(predicted:Cow) 推薦應用：IHC-P,IHC-F,ICC,IF,Flow-Cyt,ELISA

產品描述

英文名稱Nestin
中文名稱巢蛋白/神經上皮干細胞蛋白抗體
別    名ESTM 46; FLJ 21841; FLJ21841; Intermediate filament protein; Nbla00170; Nes; NEST_RAT; Nestin.  
研究領域細胞生物  發(fā)育生物學  神經生物學  干細胞  細胞類型標志物  
抗體來源Rabbit
克隆類型Polyclonal
交叉反應Human,  (predicted: Cow, )
產品應用ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test ICC=1:100 IF=1:100-500 （石蠟切片需做抗原修復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量177kDa
細胞定位細胞漿 
性    狀Liquid
濃    度1mg/ml
免 疫 原KLH conjugated synthetic peptide derived from human Nestin:21-120/1621 
亞    型IgG
純化方法affinity purified by Protein A
儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
產品介紹Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.

Function:
Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.

Subunit:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3.

Subcellular Location:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, tis protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3

Tissue Specificity:
CNS stem cells.

Post-translational modifications:
Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.

Similarity:
Belongs to the intermediate filament family.

SWISS:
P48681

Gene ID:
10763

Database links:


Entrez Gene: 10763 Human

Entrez Gene: 18008 Mouse

Entrez Gene: 25491 Rat

Omim: 600915 Human

SwissProt: P48681 Human

SwissProt: Q6P5H2 Mouse

SwissProt: P21263 Rat

Unigene: 527971 Human

Unigene: 331129 Mouse

Unigene: 9701 Rat




Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.


神經干細胞標志物
在動物體早期發(fā)育過程中，中樞神經系統(tǒng)發(fā)育尤為重要 。Nestin是一種中等纖維蛋白，它在哺乳動物神經前體細胞中表達較高，目前已被廣泛用于神經前體細胞的重要標志分子。研究Nestin的表達調控規(guī)律，將有助于人們認識神經前體細胞的生物特性。此抗體還表達中樞神經系統(tǒng)干細胞及神經上皮細胞。