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磷酸化Rho相關蛋白激酶2抗體
  • 產(chǎn)品貨號：
    BN41157R
  • 中文名稱：
    磷酸化Rho相關蛋白激酶2抗體
  • 英文名稱：
    Rabbit anti-phospho-ROCK2 (Tyr256) Polyclonal antibody
  • 品牌：
    Biorigin

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  • BN41157R-100ul

    100ul

    ￥2470.00

    交叉反應：Human,Mouse,Rat(predicted:Dog,Pig,Cow,GuineaPig) 推薦應用：IHC-P,IHC-F,IF,Flow-Cyt,ELISA

產(chǎn)品描述

英文名稱phospho-ROCK2 (Tyr256)
中文名稱磷酸化Rho相關蛋白激酶2抗體
別    名ROCK2(phospho Y256); p164 ROCK 2; Rho associated coiled coil containing protein kinase 2; Rho associated protein kinase 2; Rho kinase 2; ROCK 2; Rock II; Rock2m; ROK alpha; ROKalpha; ROCK2_HUMAN.  


產(chǎn)品類型磷酸化抗體 
研究領域免疫學  信號轉導  轉錄調(diào)節(jié)因子  
抗體來源Rabbit
克隆類型Polyclonal
交叉反應Human, Mouse, Rat,  (predicted: Dog, Pig, Cow, Guinea Pig, )
產(chǎn)品應用ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=0.2ug/test IF=1:100-500 （石蠟切片需做抗原修復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量153kDa
細胞定位細胞核 細胞漿 細胞膜 
性    狀Liquid
濃    度1mg/ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human ROCK2 around the phosphorylation site of Tyr256:PD(p-Y)IS 
亞    型IgG
純化方法affinity purified by Protein A
儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
產(chǎn)品介紹The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.

Function:
Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus.

Subunit:
Homodimer. Interacts with IRS1, RHOB and RHOC. Interacts with RHOA (activated by GTP), PPP1R12A, CHORDC1, SORL1, EP300 and BRCA2. Interacts with NPM1 and this interaction enhances its activity. Interacts with RAF1.

Subcellular Location:
Cytoplasm. Cell membrane; Peripheral membrane protein (By similarity). Nucleus. Cytoplasm, cytoskeleton, centrosome.

Post-translational modifications:
Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation at Tyr-722 reduces its binding to RHOA and is crucial for focal adhesion dynamics. Dephosphorylation by PTPN11 stimulates its RHOA binding activity.
Cleaved by granzyme B during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing.

Similarity:
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
Contains 1 REM (Hr1) repeat.

SWISS:
O75116

Gene ID:
9475

Database links:

Entrez Gene: 9475 Human

Entrez Gene: 19878 Mouse

Entrez Gene: 25537 Rat

Omim: 604002 Human

SwissProt: O75116 Human

SwissProt: P70336 Mouse



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

ROCK2（Rho kinase alpha）是ROCK1的同工酶。是一個絲/蘇氨基酸激酶，可調(diào)節(jié)細胞因子，平滑肌收縮和肌動蛋白的形成。