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磷酸化TRAF2和NCK激酶相互作用蛋白抗體
  • 產(chǎn)品貨號：
    BN41145R
  • 中文名稱：
    磷酸化TRAF2和NCK激酶相互作用蛋白抗體
  • 英文名稱：
    Rabbit anti-phospho-TNIK (Ser769) Polyclonal antibody
  • 品牌：
    Biorigin

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  • BN41145R-100ul

    100ul

    ￥2470.00

    交叉反應：Human,Mouse,Rat(predicted:Dog,Pig,Cow,Horse) 推薦應用：WB,IHC-P,IHC-F,IF,ELISA

產(chǎn)品描述

英文名稱phospho-TNIK (Ser769)
中文名稱磷酸化TRAF2和NCK激酶相互作用蛋白抗體
別    名TNIK (phospho S769); p-TNIK (phospho S769); TNIK (phospho-Ser769); TNIK (phospho-S769); p-TNIK (Ser769); p-TNIK (S769); Traf2 and NCK interacting kinase; 1500031A17RIK; 4831440I19RIK; AI451411; C530008O15Rik; C630040K21RIK; KIAA0551; MGC189819; MGC189859; RGD1561817; TNIK; TNIK_HUMAN; TRAF2 and NCK-interacting protein kinase.  
產(chǎn)品類型磷酸化抗體 
研究領(lǐng)域免疫學  信號轉(zhuǎn)導  
抗體來源Rabbit
克隆類型Polyclonal
交叉反應Human, Mouse, Rat,  (predicted: Dog, Pig, Cow, Horse, )
產(chǎn)品應用WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量150kDa
細胞定位細胞核 細胞漿 
性    狀Liquid
濃    度1mg/ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human TNIK around the phosphorylation site of Ser769:EG(p-S)PV 
亞    型IgG
純化方法affinity purified by Protein A
儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
產(chǎn)品介紹TNIK is a MSN protein kinase that interacts with both TNF receptor-associated factor 2 (TRAF2) and the adapter protein NCK. The protein has been shown to activate the c-Jun N-terminal kinase pathway when over expressed in Phoenix-A cells. TNIK has been shown to phosphorylate gelsolin, the principal intracellular and extracellular actin-severing protein, in vitro. This and evidence from mutational studies suggest that TNIK functions in the regulation of the cytoskeleton. Northern analysis indicates TNIK expression in human heart, skeletal muscle, and brain, with lower levels of expression in kidney, liver, lung, and pancreas. ESTs have been isolated from human tissue libraries, including normal amnion, gallbladder and skin.

Function:
Serine/threonine kinase that acts as an essential activator of the Wnt signaling pathway. Recruited to promoters of Wnt target genes and required to activate their expression. May act by phosphorylating TCF4/TCF7L2. Appears to act upstream of the JUN N-terminal pathway. May play a role in the response to environmental stress. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it may play a role in cytoskeletal rearrangements and regulate cell spreading. Phosphorylates SMAD1 on Thr-322.

Subunit:
Interacts (via the CNH domain) with RAP2A (GTP-bound form preferentially); the interaction is direct and required for the activation of TNIK by RAP2A. Interacts with NEDD4; recruits RAP2A to NEDD4. Interacts with TRAF2 and NCK. Interacts with TCF7L2/TCF4 and CTNNB1; the interaction is direct. Interacts with TANC1.

Subcellular Location:
Nucleus. Cytoplasm. Recycling endosome. Cytoplasm, cytoskeleton. Note=Associated with recycling endosomes and the cytoskeletal fraction upon RAP2A overexpression.

Tissue Specificity:
Expressed ubiquitously. Highest levels observed in heart, brain and skeletal muscle. Expressed in normal colonic epithelia and colorectal cancer tissues.

Post-translational modifications:
Autophosphorylated. Autophosphorylation is activated by RAP2A and induces association to the cytoskeletal fraction.

Similarity:
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CNH domain.
Contains 1 protein kinase domain.

SWISS:
Q9UKE5

Gene ID:
23043

Database links:

Entrez Gene: 23043 Human

Entrez Gene: 665113 Mouse

Omim: 610005 Human

SwissProt: Q9UKE5 Human

SwissProt: P83510 Mouse

Unigene: 34024 Human

Unigene: 126193 Mouse

Unigene: 483052 Mouse



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.