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多聚合酶α抗體
  • 產(chǎn)品貨號(hào)：
    BN41029R
  • 中文名稱：
    多聚合酶α抗體
  • 英文名稱：
    Rabbit anti-PAPOA Polyclonal antibody
  • 品牌：
    Biorigin

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  • BN41029R-100ul

    100ul

    ￥2360.00

    交叉反應(yīng)：Mouse(predicted:Human,Rat,Dog,Pig,Cow,Horse) 推薦應(yīng)用：WB,ELISA

  • BN41029R-200ul

    200ul

    ￥3490.00

    交叉反應(yīng)：Mouse(predicted:Human,Rat,Dog,Pig,Cow,Horse) 推薦應(yīng)用：WB,ELISA

產(chǎn)品描述

英文名稱PAPOA
中文名稱多聚合酶α抗體
別    名MGC5378; PAP alpha; PAP-alpha; PAP; PapIII; Plap; poly(A) polymerase alpha; Polynucleotide adenylyltransferase alpha.  
抗體來源Rabbit
克隆類型Polyclonal
交叉反應(yīng)Mouse,  (predicted: Human, Rat, Dog, Pig, Cow, Horse, )
產(chǎn)品應(yīng)用WB=1:500-2000 ELISA=1:5000-10000 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量82kDa
細(xì)胞定位細(xì)胞核 細(xì)胞漿 
性    狀Liquid
濃    度1mg/ml
免 疫 原KLH conjugated synthetic peptide derived from human PAPOA:601-700/745 
亞    型IgG
純化方法affinity purified by Protein A
儲(chǔ) 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
產(chǎn)品介紹he protein encoded by this gene belongs to the poly(A) polymerase family. It is required for the addition of adenosine residues for the creation of the 3'-poly(A) tail of mRNAs. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011].

Function:
Polymerase that creates the 3'-poly(A) tail of mRNA's. Also required for the endoribonucleolytic cleavage reaction at some polyadenylation sites. May acquire specificity through interaction with a cleavage and polyadenylation specificity factor (CPSF) at its C-terminus.

Subunit:
Monomer. Found in a complex with CPSF1, FIP1L1 and PAPOLA. Interacts with FIP1L1 (By similarity). Interacts with NUDT21; the interaction is diminished by acetylation. Interacts with KPNB1; the interaction promotes PAP nuclear import and is inhibited by acetylation of PAP.

Subcellular Location:
Cytoplasm. Nucleus. Note=The 90 kDa form is nuclear while the 100 kDa and the 106 kDa forms are both nuclear and cytoplasmic.

Post-translational modifications:
Polysumoylated. Varying sumolyation depending on tissue- and cell-type. Highly sumoylated in bladder and NIH 3T3 cells. Sumoylation is required for nuclear localization and enhances PAP stability. Desumoylated by SENP1. Inhibits polymerase activity.
Hyperphosphorylation on multiple CDK2 consensus and non-consensus sites in the C-terminal Ser/Thr-rich region represses PAP activity in late M-phase. Phosphorylation/dephosphorylation may regulate the interaction between PAP and CPSF.
Acetylated in the C-terminus. Acetylation decreases interaction with NUDT21 and KPNB1, and inhibits nuclear localization through inhibiting binding to the importin alpha/beta complex.

Similarity:
Belongs to the poly(A) polymerase family.

SWISS:
P51003

Gene ID:
10914

Database links:

Entrez Gene: 10914 Human

Entrez Gene: 18789 Mouse

Entrez Gene: 314417 Rat

Omim: 605553 Human

SwissProt: P51003 Human

SwissProt: Q61183 Mouse



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.


















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