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熱休克因子1抗體
  • 產(chǎn)品貨號：
    BN40955R
  • 中文名稱：
    熱休克因子1抗體
  • 英文名稱：
    Rabbit anti-HSF1 Polyclonal antibody
  • 品牌：
    Biorigin

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    產(chǎn)品規(guī)格

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  • BN40955R-100ul

    100ul

    ￥2360.00

    交叉反應：Rat,Mouse,Human(predicted:Rabbit,Cow,Pig,Dog) 推薦應用：WB,IHC-P,IHC-F,ICC,IF,ELISA

  • BN40955R-200ul

    200ul

    ￥3490.00

    交叉反應：Rat,Mouse,Human(predicted:Rabbit,Cow,Pig,Dog) 推薦應用：WB,IHC-P,IHC-F,ICC,IF,ELISA

產(chǎn)品描述

英文名稱HSF1
中文名稱熱休克因子1抗體
別    名Heat shock factor 1; Heat shock factor protein 1; Heat shock transcription factor 1; HSF 1; hsf1; HSTF 1; HSTF1; HSF1_HUMAN.  
研究領(lǐng)域腫瘤  細胞生物  免疫學  信號轉(zhuǎn)導  細胞凋亡  轉(zhuǎn)錄調(diào)節(jié)因子  
抗體來源Rabbit
克隆類型Polyclonal
交叉反應Human, Mouse, Rat,  (predicted: Dog, Pig, Cow, Rabbit, )
產(chǎn)品應用WB=1:200-500 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量57kDa
細胞定位細胞核 細胞漿 
性    狀Liquid
濃    度1mg/ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human HSF1 around the phosphorylation site of Ser303:351-450/529 
亞    型IgG
純化方法affinity purified by Protein A
儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
產(chǎn)品介紹The product of this gene is a heat-shock transcription factor. Transcription of heat-shock genes is rapidly induced after temperature stress. Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of this gene. [provided by RefSeq, Jul 2008].

Function:
DNA-binding protein that specifically binds heat shockpromoter elements (HSE) and activates transcription. In highereukaryotes, HSF is unable to bind to the HSE unless the cells areheat shocked.

Subunit:
Monomer. Under normal conditions, interacts with HSP90AA1in the HSP90 multichaperone complex; the interaction preventstrimerization and activation of HSF1. On activation by heat-stressor by other factors such as metal ions, HSF1 is released from thecomplex, homotrimerizes, is hyperphosphorylated and translocated tothe nucleus where, subsequently, it can activate transcription.Binds the complex through the regulatory domain. Interacts withSYMPK and CSTF2 in heat-stressed cells. Interacts with FKBP4 in theHSP90 multichaperone complex; the interaction is independent of thephosphorylation state of HSF1. Interacts with MAPKAPK2.

Subcellular Location:
Cytoplasm. Nucleus. Note=Cytoplasmic duringnormal growth. On activation, translocates to nuclear stressgranules. Colocalizes with SUMO1 in nuclear stress granules.

Tissue Specificity:
Phosphorylated on multiple serine residues, a subset of whichare involved in stress-related regulation of transcriptionactivation. Constitutive phosphorylation represses transcriptionalactivity at normal temperatures. Levels increase on specificresidues heat-shock and enhance HSF1 transactivation activity.Phosphorylation on Ser-307 derepresses activation on heat-stressand in combination with Ser-303 phosphorylation appears to beinvolved in recovery after heat-stress. Phosphorylated on Ser-230by CAMK2, in vitro. Cadmium also enhances phosphorylation at thissite. Phosphorylation on Ser-303 is a prerequisite for HSF1sumoylation. Phosphorylation on Ser-121 inhibits transactivationand promotes HSP90 binding. Phosphorylation on Thr-142 alsomediates transcriptional activity induced by heat.
Sumoylated with SUMO1 and SUMO2 on heat-shock. Heat-induciblesumoylation occurs after 15 min of heat-shock, after which levelsdecrease and at 4 hours, levels return to control levels.Sumoylation has no effect on HSE binding nor on transcriptionalactivity. Phosphorylation on Ser-303 is a prerequisite forsumoylation.

Similarity:
Belongs to the HSF family.

SWISS:
Q00613

Gene ID:
3297

Database links:

Entrez Gene: 3297 Human

Entrez Gene: 15499 Mouse

Entrez Gene: 79245 Rat

Omim: 140580 Human

SwissProt: Q00613 Human

SwissProt: P38532 Mouse

Unigene: 530227 Human

Unigene: 347444 Mouse



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.





















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