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磷酸化核酸酶敏感元件結(jié)合蛋白1抗體
  • 產(chǎn)品貨號(hào)：
    BN40930R
  • 中文名稱：
    磷酸化核酸酶敏感元件結(jié)合蛋白1抗體
  • 英文名稱：
    Rabbit anti-Phospho-YB1 (Ser102) Polyclonal antibody
  • 品牌：
    Biorigin

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  • BN40930R-100ul

    100ul

    ￥2470.00

    交叉反應(yīng)：Human,Mouse,Rat(predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit,GuineaPig) 推薦應(yīng)用：WB,IHC-P,IHC-F,ICC,IF,ELISA

產(chǎn)品描述

英文名稱Phospho-YB1 (Ser102)
中文名稱磷酸化核酸酶敏感元件結(jié)合蛋白1抗體
別    名Y box-binding protein1; nuclease sensitive element binding protein 1; YB1; BP-8; CSDB; DBPB; YB-1; CSDA2; NSEP1; NSEP-1; MDR-NF1; MGC104858; MGC110976; MGC117250; YBX1; CCAAT binding transcription factor I subunit A; DNA binding protein B; major histocompatibility complex, class II, Y box-binding protein I; CBF-A; EFI-A; Enhancer factor I subunit A; EFI-A; p50; Q15905; YBOX1_HUMAN.  
產(chǎn)品類型磷酸化抗體 
研究領(lǐng)域腫瘤  細(xì)胞生物  免疫學(xué)  信號(hào)轉(zhuǎn)導(dǎo)  轉(zhuǎn)錄調(diào)節(jié)因子  
抗體來源Rabbit
克隆類型Polyclonal
交叉反應(yīng)Human,  (predicted: Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Guinea Pig, )
產(chǎn)品應(yīng)用WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量36kDa
細(xì)胞定位細(xì)胞核 細(xì)胞漿 分泌型蛋白 
性    狀Liquid
濃    度1mg/ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human YB1 around the phosphorylation site of Ser102:LR(p-S)VG 
亞    型IgG
純化方法affinity purified by Protein A
儲(chǔ) 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
產(chǎn)品介紹The Y-box binding protein 1(YB1)is a pluripotent DNA/RNA-binding factor which regulates gene expression through transcription and translation. YB1 has been shown to be a marker of tumour aggressiveness and belongs to the cold-shock domain family.

Function:
Mediates pre-mRNA alternative splicing regulation. Binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors (By similarity). Regulates the transcription of numerous genes. Its transcriptional activity on the multidrug resistance gene MDR1 is enhanced in presence of the APEX1 acetylated form at 'Lys-6' and 'Lys-7'. Binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as MDR1 and HLA class II genes. Promotes separation of DNA strands that contain mismatches or are modified by cisplatin. Has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA (in vitro). May play a role in DNA repair. Component of the CRD-mediated complex that promotes MYC mRNA stability. Binds preferentially to the 5'-[CU]CUGCG-3' motif in vitro.
The secreted form acts as an extracellular mitogen and stimulates cell migration and proliferation.

Subunit:
Identified in a histone pre-mRNA complex, at least composed of ERI1, LSM11, SLBP, SNRPB, SYNCRIP and YBX1 (By similarity). Component of the coding region determinant (CRD)-mediated complex, composed of DHX9, HNRNPU, IGF2BP1, SYNCRIP and YBX1. Identified in a mRNP complex, at least composed of DHX9, DDX3X, ELAVL1, HNRNPU, IGF2BP1, ILF3, PABPC1, PCBP2, PTBP2, STAU1, STAU2, SYNCRIP and YBX1. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Component of the U11/U12 snRNPs that are part of the U12-type spliceosome. Interacts with IGF2BP1 and RBBP6. Component of cytoplasmic messenger ribonucleoprotein particles (mRNPs). Interacts with AKT1, MBNL1, SFRS9, ALYREF/THOC4, MSH2, XRCC5, WRN and NCL. Can bind to DNA as a homomeric form, (EFI-A)n or as a heteromeric form in association with EFI-B. Homodimer in the presence of ATP. Interacts (via C-terminus) with APEX1 (via N-terminus); the interaction is increased with APEX1 acetylated at 'Lys-6' and 'Lys-7'. Interacts with EIF2C1 and EIF2C2.

Subcellular Location:
Cytoplasm. Nucleus. Cytoplasmic granule. Secreted. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles between nucleus and cytoplasm. Predominantly cytoplasmic in proliferating cells. Cytotoxic stress and DNA damage enhance translocation to the nucleus. Localized with DDX1, MBNL1 and TIAL1 in stress granules upon stress. Secreted by mesangial and monocytic cells after inflammatory challenges. Translocates from the cytoplasm to the nucleus after and colocalizes with APEX1 in nuclear speckles after genotoxic stress.

Post-translational modifications:
Ubiquitinated by RBBP6; leading to a decrease of YBX1 transcactivational ability.
In the absence of phosphorylation the protein is retained in the cytoplasm.br<cleaved by="" a="" 20s="" proteasomal="" protease="" in="" response="" to="" agents="" that="" damage="" dna.="" cleavage="" takes="" place="" the="" absence="" of="" ubiquitination="" and="" atp.="" resulting="" n-terminal="" fragment="" accumulates="" nucleus="" (by="" similarity).
Similarity:
Contains 1 CSD (cold-shock) domain.

SWISS:
P67809

Gene ID:
4904

Database links:

Entrez Gene: 4904 Human

Entrez Gene: 22608 Mouse

Entrez Gene: 500538 Rat

Omim: 154030 Human

SwissProt: P67809 Human

SwissProt: P62960 Mouse

SwissProt: P62961 Rat

Unigene: 473583 Human

Unigene: 258204 Mouse

Unigene: 110976 Rat

Unigene: 194909 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.