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磷酸化CREB轉錄共激活因子TORC2抗體
  • 產(chǎn)品貨號：
    BN40913R
  • 中文名稱：
    磷酸化CREB轉錄共激活因子TORC2抗體
  • 英文名稱：
    Rabbit anti-Phospho-TORC2 (Ser171) Polyclonal antibody
  • 品牌：
    Biorigin

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  • BN40913R-100ul

    100ul

    ￥2470.00

    交叉反應：Human,Mouse(predicted:Rat,Dog,Pig) 推薦應用：WB,Flow-Cyt,ELISA

產(chǎn)品描述

英文名稱Phospho-TORC2 (Ser171)
中文名稱磷酸化CREB轉錄共激活因子TORC2抗體
別    名TORC2 (phospho S171); TORC2 (phospho Ser171); p-TORC2 (Ser171); CREB regulated transcription coactivator 2; CRTC2; RP11-422P24.6; TORC-2; Transducer of CREB protein 2; Transducer of regulated cAMP response element-binding protein; Transducer of regulated cAMP response element-binding protein (CREB) 2; Transducer of regulated cAMP response element-binding protein 2; Transducer of regulated CREB protein 2; CRTC2_HUMAN. 




產(chǎn)品類型磷酸化抗體 
研究領域腫瘤  免疫學  信號轉導  細胞凋亡  轉錄調(diào)節(jié)因子  激酶和磷酸酶  
抗體來源Rabbit
克隆類型Polyclonal
交叉反應Human, Mouse,  (predicted: Rat, Dog, Pig, )
產(chǎn)品應用WB=1:500-2000 ELISA=1:5000-10000 Flow-Cyt=1ug/Test 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量73kDa
細胞定位細胞核 細胞漿 
性    狀Liquid
濃    度1mg/ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human CREB regulated transcription coactivator 2 around the phosphorylation site of Ser171:TS(p-S)DS 
亞    型IgG
純化方法affinity purified by Protein A
儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
產(chǎn)品介紹This gene encodes a member of the transducers of regulated cAMP response element-binding protein activity family of transcription coactivators. These proteins promote the transcription of genes targeted by the cAMP response element-binding protein, and therefore play an important role in many cellular processes. Under basal conditions the encoded protein is phosphorylated by AMP-activated protein kinase or the salt-inducible kinases and is sequestered in the cytoplasm. Upon activation by elevated cAMP or calcium, the encoded protein translocates to the nucleus and increases target gene expression. Single nucleotide polymorphisms in this gene may increase the risk of type 2 diabetes. A pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Dec 2010].

Function:
Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. Regulates the expression of specific genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR).

Subunit:
Binds, as a tetramer, through its N-terminal region, with the bZIP domain of CREB1. 'Arg-314' in the bZIP domain of CREB1 is essential for this interaction. Interaction, via its C-terminal, with TAF4, enhances recruitment of TAF4 to CREB1. Interacts with PPP3CA/calcineurin alpha, SIK2 and 14-3-3 proteins, YWHAB and YWHAG. Interaction with the human T-cell leukemia virus type 1 (HTLV-1) Tax protein is essential for optimal transcription activation by Tax. Interaction with RFWD2/COP1 mediates nuclear export and degradation of CRTC2.

Subcellular Location:
Cytoplasm. Nucleus. Note=Translocated from the nucleus to the cytoplasm on interaction of the phosphorylated form with 14-3-3 protein. In response to cAMP levels and glucagon, relocated to the nucleus.

Tissue Specificity:
Most abundantly expressed in the thymus. Present in both B and T-lymphocytes. Highly expressed in HEK293T cells and in insulinomas. High levels also in spleen, ovary, muscle and lung, with highest levels in muscle. Lower levels found in brain, colon, heart, kidney, prostate, small intestine and stomach. Weak expression in liver and pancreas.

Post-translational modifications:
Phosphorylation/dephosphorylation states of Ser-171 are required for regulating transduction of CREB activity. TORCs are inactive when phosphorylated, and active when dephosphorylated at this site. This primary site of phosphorylation, is regulated by cAMP and calcium levels and is dependent on the phosphorylation of SIKs (SIK1 and SIK2) by LKB1. Both insulin and AMPK increase this phosphorylation of CRTC2 while glucagon suppresses it. Phosphorylation at Ser-274 by MARK2 is induced under low glucose conditions and dephosphorylated in response to glucose influx. Phosphorylation at Ser-274 promotes interaction with 14-3-3 proteins and translocation to the cytoplasm.

Similarity:
Belongs to the TORC family.

SWISS:
Q53ET0

Gene ID:
200186

Database links:

Entrez Gene: 200186 Human

Entrez Gene: 74343 Mouse

Entrez Gene: 310615 Rat

Omim: 608972 Human

SwissProt: Q53ET0 Human

SwissProt: Q3U182 Mouse

SwissProt: Q3LRZ1 Rat

Unigene: 406392 Human

Unigene: 35627 Mouse

Unigene: 13599 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

CREB轉錄共激活因子TORC2又稱葡萄糖關鍵調(diào)控蛋白TORC-2。