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磷酸化周期素E1抗體
  • 產(chǎn)品貨號：
    BN40863R
  • 中文名稱：
    磷酸化周期素E1抗體
  • 英文名稱：
    Rabbit anti-phospho-Cyclin E1 (Thr77) Polyclonal antibody
  • 品牌：
    Biorigin

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  • BN40863R-100ul

    100ul

    ￥2470.00

    交叉反應(yīng)：Human(predicted:Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit) 推薦應(yīng)用：WB,Flow-Cyt,ELISA

產(chǎn)品描述

英文名稱phospho-Cyclin E1 (Thr77)
中文名稱磷酸化周期素E1抗體
別    名Cyclin E1 (phospho-Thr77); Cyclin E1 (phospho-T77); CCNE 1; CCNE; CCNE1; Cyclin Es; Cyclin Et; CyclinE; G1/S specific cyclin E; G1/S-specific cyclin-E1; CCNE1_HUMAN; pCCNE1.  
產(chǎn)品類型磷酸化抗體 
研究領(lǐng)域腫瘤  細(xì)胞生物  細(xì)胞周期蛋白  
抗體來源Rabbit
克隆類型Polyclonal
交叉反應(yīng)Human,  (predicted: Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, )
產(chǎn)品應(yīng)用WB=1:500-2000 ELISA=1:5000-10000 Flow-Cyt=1μg /Test 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量47kDa
細(xì)胞定位細(xì)胞核 
性    狀Liquid
濃    度1mg/ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human Cyclin E around the phosphorylation site of Thr77:IP(p-T)PD 
亞    型IgG
純化方法affinity purified by Protein A
儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
產(chǎn)品介紹Cyclin E is a regulatory subunit of Cdk2 and controls G1 / S transition during the mammalian cell cycle. Multiple isoforms of Cyclin E are only expressed in tumors but not in normal tissue, suggesting a post transcriptional regulation of Cyclin E. In vitro analyses indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1. Alterations in the Cyclin E protein have been implicated as indicators of worse prognosis in various cancers.

Function:
Essential for the control of the cell cycle at the G1/S (start) transition.

Subunit:
Interacts with a member of the CDK2/CDK protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Found in a complex with CDK2, CABLES1 and CCNA1 (By similarity). Part of a complex consisting of UHRF2, CDK2 and CCNE1. Interacts directly with UHRF2; the interaction ubiquitinates CCNE1 and appears to occur independently of CCNE1 phosphorylation.

Subcellular Location:
Nucleus.

Tissue Specificity:
Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.

Post-translational modifications:
Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated by UHRF2; appears to occur independently of phosphorylation.

Similarity:
Belongs to the cyclin family. Cyclin E subfamily.

SWISS:
P24864

Gene ID:
898

Database links:

Entrez Gene: 898 Human

Entrez Gene: 12447 Mouse

Entrez Gene: 25729 Rat

Omim: 123837 Human

SwissProt: P24864 Human

SwissProt: Q61457 Mouse

SwissProt: P39949 Rat

Unigene: 244723 Human

Unigene: 16110 Mouse

Unigene: 15455 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

    細(xì)胞周期素E是調(diào)控細(xì)胞G-1→S期轉(zhuǎn)變的關(guān)鍵因素。由于在多種腫瘤中的不適當(dāng)表達(dá),細(xì)胞周期素E現(xiàn)在已明確為原癌基因。