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磷酸化腺苷單磷酸活化蛋白激酶β1抗體
  • 產(chǎn)品貨號：
    BN40848R
  • 中文名稱：
    磷酸化腺苷單磷酸活化蛋白激酶β1抗體
  • 英文名稱：
    Rabbit anti-phospho-AMPK beta 1 + AMPK beta 2 (Ser182/Ser184) Polyclonal antibody
  • 品牌：
    Biorigin

  • 貨號

    產(chǎn)品規(guī)格

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  • BN40848R-100ul

    100ul

    ￥2470.00

    交叉反應(yīng)：Mouse,Rat(predicted:Human,Dog,Cow,Horse,Rabbit) 推薦應(yīng)用：WB,IHC-P,IHC-F,IF,Flow-Cyt,ELISA

產(chǎn)品描述

英文名稱phospho-AMPK beta 1 + AMPK beta 2 (Ser182/Ser184)
中文名稱磷酸化腺苷單磷酸活化蛋白激酶β1抗體
別    名phospho-AMPK beta 1 + AMPK beta 2 (Ser182+Ser184); PRKAB1(phospho S182); PRKAB1(phospho-S182); AMPK beta 1(phospho Ser182); p-AMPK beta 1(Ser182); p-AMPK beta 1(S182); AMPK beta 2(phospho Ser184); 5 AMP activated protein kinase subunit beta 1; AMPK; AMPK beta 1 chain; AMPKb; HAMPKb; PRKAB1; 5'-AMP-activated protein kinase subunit beta-1; AMP-activated protein kinase beta subunit; protein kinase, AMP-activated, noncatalytic, beta-1; AMPK beta -1 chain; 5'-AMP-activated protein kinase beta-1 subunit; AMPKb; AMPK subunit beta-1; AAKB1_HUMAN; AMPK b1; AMPK-b1.  
產(chǎn)品類型磷酸化抗體 
研究領(lǐng)域腫瘤  細(xì)胞生物  信號轉(zhuǎn)導(dǎo)  細(xì)胞凋亡  轉(zhuǎn)錄調(diào)節(jié)因子  激酶和磷酸酶  
抗體來源Rabbit
克隆類型Polyclonal
交叉反應(yīng)Mouse, Rat,  (predicted: Human, Dog, Cow, Horse, Rabbit, )
產(chǎn)品應(yīng)用WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2ug/Test IF=1:100-500 （石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量30kDa
細(xì)胞定位細(xì)胞核 細(xì)胞漿 
性    狀Liquid
濃    度1mg/ml
免 疫 原KLH conjugated synthesised phosphopeptide derived from human AMPK beta 1 around the phosphorylation site of Ser182:SS(p-S)PP 
亞    型IgG
純化方法affinity purified by Protein A
儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
產(chǎn)品介紹The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex. [provided by RefSeq, Jul 2008].

Function:
Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).

Subunit:
AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3). Interacts with FNIP1 and FNIP2.

Tissue Specificity:
Highly expressed in kidney, heart, white adipose tissue, lung and spleen.

Post-translational modifications:
Phosphorylated when associated with the catalytic subunit (PRKAA1 or PRKAA2). Phosphorylated by ULK1; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1 and AMPK.

Similarity:
Belongs to the 5'-AMP-activated protein kinase beta subunit family.

SWISS:
Q9Y478

Gene ID:
5564

Database links:

Entrez Gene: 5564 Human

Entrez Gene: 19079 Mouse

Entrez Gene: 83803 Rat

Omim: 602740 Human

SwissProt: Q9Y478 Human

SwissProt: Q9R078 Mouse

SwissProt: P80386 Rat

Unigene: 6061 Human

Unigene: 726001 Human

Unigene: 458152 Mouse

Unigene: 3619 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

AMPKβ1(AMP-activated Protein Kinase beta-1)(腺苷單磷酸活化蛋白激酶β-1)是一種參與細(xì)胞適應(yīng)能量危機的應(yīng)激反應(yīng)酶,AMPK不僅可以在細(xì)胞水平作為能量的感受器，還可以通過激素和細(xì)胞因子，如瘦素、脂聯(lián)素和ghrelin來參與調(diào)節(jié)機體的能量消耗和能量攝入.