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首頁>>免疫學>>一抗>>絲氨酸/蘇氨酸蛋白激酶MLK3抗體
絲氨酸/蘇氨酸蛋白激酶MLK3抗體
  • 產(chǎn)品貨號：
    BN40841R
  • 中文名稱：
    絲氨酸/蘇氨酸蛋白激酶MLK3抗體
  • 英文名稱：
    Rabbit anti-MLK3 Polyclonal antibody
  • 品牌：
    Biorigin

  • 貨號

    產(chǎn)品規(guī)格

    售價

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  • BN40841R-100ul

    100ul

    ￥2360.00

    交叉反應(yīng)：Human,Mouse,Rat(predicted:Cow,Horse) 推薦應(yīng)用：WB,IHC-P,IHC-F,IF,ELISA

  • BN40841R-200ul

    200ul

    ￥3490.00

    交叉反應(yīng)：Human,Mouse,Rat(predicted:Cow,Horse) 推薦應(yīng)用：WB,IHC-P,IHC-F,IF,ELISA

產(chǎn)品描述

英文名稱MLK3
中文名稱絲氨酸/蘇氨酸蛋白激酶MLK3抗體
別    名MEKK11; MGC17114; Mitogen-activated protein kinase kinase kinase 11; Mixed lineage kinase 3; Mixed lineage protein kinase 3; Mlk-3; Mlk3; Protein tyrosine kinase PTK1; PTK1; RHOE; SH3 domain containing proline rich kinase; SPRK; Src-homology 3 domain-containing proline-rich kinase; 2610017K16Rik; EC 2.7.11.25; M3K11_HUMAN.  
研究領(lǐng)域腫瘤  免疫學  神經(jīng)生物學  信號轉(zhuǎn)導  細胞凋亡  轉(zhuǎn)錄調(diào)節(jié)因子  激酶和磷酸酶  
抗體來源Rabbit
克隆類型Polyclonal
交叉反應(yīng)Human, Mouse, Rat,  (predicted: Cow, Horse, )
產(chǎn)品應(yīng)用WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量93kDa
細胞定位細胞漿 
性    狀Liquid
濃    度1mg/ml
免 疫 原KLH conjugated synthetic peptide derived from human MLK3:355-460/847 
亞    型IgG
純化方法affinity purified by Protein A
儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
產(chǎn)品介紹Members of the mixed-lineage kinase (MLK) family (including MLK1, MLK2, MLK3, and dual leucine zipper kinase [DLK]) are serine/threonine protein kinases that are expressed in multiple cell types. MLK3 is activated by phosphorylation in response to stress stimuli (e.g., inflammatory responses, UV, chemical stress) that are coupled to the small GTPase, Cdc42/rac. MLK3 is a multifunctional kinase that plays an essential role in several signaling pathways, including mitogen-activated protein kinase (i.e. activation of JNK and p38), IkappaB/NFkappaB, and p70 S6 kinase. Indeed MLK3 signaling occurs through multiple signaling domains in this protein kinase including (from N- to C-terminal) a glycine-rich domain, Src homology 3 (SH3) domain, a kinase domain, a zipper domain, a Cdc42/rac interactive binding (CRIB) domain and a Pro/Ser/Thr-rich domain. Phosphorylation of MLK3 occurs on multiple residues including threonine 277 and serine 281 within the activation loop of the kinase domain.

Function:
Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1). Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle.

Subunit:
Homodimer; undergoes dimerization during activation.

Subcellular Location:
Cytoplasm, cytoskeleton, centrosome. Note=Location is cell cycle dependent.

Tissue Specificity:
Expressed in a wide variety of normal and neoplastic tissues including fetal lung, liver, heart and kidney, and adult lung, liver, heart, kidney, placenta, skeletal muscle, pancreas and brain.

Post-translational modifications:
Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-277 is likely to be the main autophosphorylation site. Phosphorylation of Ser-555 and Ser-556 is induced by CDC42.

Similarity:
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain.
Contains 1 SH3 domain.

SWISS:
Q16584

Gene ID:
4296

Database links:

Entrez Gene: 4296 Human

Entrez Gene: 26403 Mouse

Omim: 600050 Human

SwissProt: Q16584 Human

SwissProt: Q6P2G4 Human

SwissProt: Q80XI6 Mouse

Unigene: 502872 Human

Unigene: 185026 Mouse



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

MLK3是JNK蛋白上游的重要活化因子，通過直接磷酸化并激活中游MKK4／7蛋白激酶，從而激活JNK應(yīng)激信號通路。