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磷酸化真核翻譯起始因子4E抗體
  • 產(chǎn)品貨號：
    BN40817R
  • 中文名稱：
    磷酸化真核翻譯起始因子4E抗體
  • 英文名稱：
    Rabbit anti-phospho-eIF4E (Ser209) Polyclonal antibody
  • 品牌：
    Biorigin

  • 貨號

    產(chǎn)品規(guī)格

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  • BN40817R-100ul

    100ul

    ￥2470.00

    交叉反應：Human,Mouse,Rat,Zebrafish(predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit) 推薦應用：WB,IHC-P,IHC-F,IF,Flow-Cyt,ELISA

產(chǎn)品描述

英文名稱phospho-eIF4E (Ser209)
中文名稱磷酸化真核翻譯起始因子4E抗體
別    名eIF4E(phospho S209); eIF4E(phospho Ser209); p-eIF4E(Ser209); EIF4F; eIF-4E; eIF 4E; CBP; eIF 4F 25 kDa subunit; CBP; eIF 4E; eIF 4F 25 kDa subunit; EIF 4F; EIF4E1; EIF4EL1; EIF4F; Eukaryotic Translation Initiation Factor 4 E; Eukaryotic translation initiation factor 4E like 1; Messanger RNA Cap Binding Protein eIF 4E; IF4E_HUMAN.  
產(chǎn)品類型磷酸化抗體 
研究領域免疫學  信號轉導  細胞凋亡  轉錄調節(jié)因子  
抗體來源Rabbit
克隆類型Polyclonal
交叉反應Human, Mouse, Rat,  (predicted: Chicken, Dog, Pig, Cow, Horse, Rabbit, Daniorerio)
產(chǎn)品應用WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=0.2ug/test IF=1:100-500 （石蠟切片需做抗原修復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量24kDa
細胞定位細胞核 細胞漿 
性    狀Liquid
濃    度1mg/ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human eIF4E around the phosphorylation site of Ser209:SG(p-S)TT 
亞    型IgG
純化方法affinity purified by Protein A
儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
產(chǎn)品介紹eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types. Several kinases are currently being investigated as potential regulators of eIF4E including PKC and/or the MAP kinase activated Mnk.

Function:
Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.

Subunit:
eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4E is also known to interact with other partners. The interaction with EIF4ENIF1 mediates the import into the nucleus. Nonphosphorylated EIF4EBP1, EIF4EBP2 and EIF4EBP3 compete with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Rapamycin can attenuate insulin stimulation, mediated by FKBPs. Interacts mutually exclusive with EIF4A1 or EIF4A2. Interacts with NGDN and PIWIL2. Component of the CYFIP1-EIF4E-FMR1 complex composed of CYFIP, EIF4E and FMR1. Interacts directly with CYFIP1 (By similarity). Interacts with Lassa virus Z protein. Binds to MKNK2 in nucleus. Interacts with LIMD1, WTIP and AJUBA.

Subcellular Location:
Cytoplasm, P-body.

Post-translational modifications:
Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.

Similarity:
Belongs to the eukaryotic initiation factor 4E family.

SWISS:
P06730

Gene ID:
1977

Database links:

Entrez Gene: 1977 Human

Entrez Gene: 13684 Mouse

Entrez Gene: 117045 Rat

Omim: 133440 Human

SwissProt: P06730 Human

SwissProt: P63073 Mouse

SwissProt: P63074 Rat

Unigene: 249718 Human

Unigene: 3941 Mouse

Unigene: 11275 Rat





Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

EIF4E是蛋白合成的一個主要調控因子，它表達量增加會促進多種腫瘤的生長的增值、分化，因此EIF4E是一種非常重要的抗癌藥物靶標。