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端粒酶逆轉(zhuǎn)錄酶抗體
  • 產(chǎn)品貨號(hào)：
    BN40650R
  • 中文名稱：
    端粒酶逆轉(zhuǎn)錄酶抗體
  • 英文名稱：
    Rabbit anti-TERT Polyclonal antibody
  • 品牌：
    Biorigin

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  • BN40650R-100ul

    100ul

    ￥2360.00

    交叉反應(yīng)：Human,Mouse(predicted:Rat,Dog) 推薦應(yīng)用：WB,ICC,Flow-Cyt,ELISA

  • BN40650R-200ul

    200ul

    ￥3490.00

    交叉反應(yīng)：Human,Mouse(predicted:Rat,Dog) 推薦應(yīng)用：WB,ICC,Flow-Cyt,ELISA

產(chǎn)品描述

英文名稱TERT
中文名稱端粒酶逆轉(zhuǎn)錄酶抗體
別    名EST2; hEST2; TCS1; Telomerase associated protein 2; Telomerase Catalytic Subunit; Telomere Reverse Transcriptase; Telomerase reverse transcriptase; TERT; TP2; TRT; TERT_HUMAN; Telomerase reverse transcriptase; HEST2; Telomerase catalytic subunit; Telomerase-associated protein 2.  
研究領(lǐng)域腫瘤  細(xì)胞生物  免疫學(xué)  細(xì)胞凋亡  轉(zhuǎn)錄調(diào)節(jié)因子  
抗體來(lái)源Rabbit
克隆類型Polyclonal
交叉反應(yīng)Human, Mouse,  (predicted: Rat, Dog, )
產(chǎn)品應(yīng)用WB=1:500-2000 ELISA=1:5000-10000 Flow-Cyt=1μg/Test ICC=1:100 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量124kDa
細(xì)胞定位細(xì)胞核 細(xì)胞漿 
性    狀Liquid
濃    度1mg/ml
免 疫 原KLH conjugated synthetic peptide derived from human TERT:521-620/1132 
亞    型IgG
純化方法affinity purified by Protein A
儲(chǔ) 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
產(chǎn)品介紹Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. It elongates telomeres. It is a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. Telomerase are large DNA-protein complexes with telomerase expression being the subject of recent research due to its link to cell immortalization. Recent evidence has shown that MYC upregulates the catalytic subunit of telomerase, TERT, and that Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced variants encoding different isoforms of telomerase reverse transcriptase have been identified; the full-length sequence of some variants has not been determined. Alternative splicing at this locus is thought to be one mechanism of regulation of telomerase activity. [provided by RefSeq, Jul 2008].

Function:
Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somatic cells. Catalytic component of the teleromerase holoenzyme complex whose main activity is the elongation of telomeres by acting as a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. Catalyzes the RNA-dependent extension of 3'-chromosomal termini with the 6-nucleotide telomeric repeat unit, 5'-TTAGGG-3'. The catalytic cycle involves primer binding, primer extension and release of product once the template boundary has been reached or nascent product translocation followed by further extension. More active on substrates containing 2 or 3 telomeric repeats. Telomerase activity is regulated by a number of factors including telomerase complex-associated proteins, chaperones and polypeptide modifiers. Modulates Wnt signaling. Plays important roles in aging and antiapoptosis.

Subunit:
Homodimer; dimerization is required to produce a functional complex. Oligomer; can form oligomers in the absence of the telomerase RNA template component (TERC). Catalytic subunit of the telomerase holoenzyme complex composed minimally of TERT and TERC. The telomerase complex is composed of TERT, DKC1, WDR79/TCAB1, NOP10, NHP2, GAR1, TEP1, EST1A, POT1 and a telomerase RNA template component (TERC). The molecular chaperone HSP90/P23 complex is required for correct assembly and stabilization of the active telomerase. Interacts directly with HSP90A and PTGES3. Interacts with HSPA1A; the interaction occurs in the absence of TERC and dissociates once the complex has formed. Interacts with RAN; the interaction promotes nuclear export of TERT. Interacts with XPO1. Interacts with PTPN11; the interaction retains TERT in the nucleus. Interacts with NCL (via RRM1 and C-terminal RRM4/Arg/Gly-rich domains); the interaction is important for nucleolar localization of TERT. Interacts with SMARCA4 (via the bromodomain); the interaction regulates Wnt-mediated signaling. Interacts with MCRS1 (isoform MCRS2); the interaction inhibits in vitro telomerase activity. Interacts with PIF1; the interaction has no effect on the elongation activity of TERT. Interacts with PML; the interaction recruits TERT to PML bodies and inhibits telomerase activity.

Subcellular Location:
Nucleus, nucleolus. Nucleus, nucleoplasm. Nucleus. Chromosome, telomere. Cytoplasm. Nucleus, PML body. Note=Shuttling between nuclear and cytoplasm depends on cell cycle, phosphorylation states, transformation and DNA damage. Diffuse localization in the nucleoplasm. Enriched in nucleoli of certain cell types. Translocated to the cytoplasm via nuclear pores in a CRM1/RAN-dependent manner involving oxidative stress-mediated phosphorylation at Tyr-707. Dephosphorylation at this site by SHP2 retains TERT in the nucleus. Translocated to the nucleus by phosphorylation by AKT.

Tissue Specificity:
Expressed at a high level in thymocyte subpopulations, at an intermediate level in tonsil T-lymphocytes, and at a low to undetectable level in peripheral blood T-lymphocytes.

Post-translational modifications:
Ubiquitinated, leading to proteasomal degradation.
Phosphorylation at Tyr-707 under oxidative stress leads to translocation of TERT to the cytoplasm and reduces its antiapoptotic activity. Dephosphorylated by SHP2/PTPN11 leading to nuclear retention. Phosphorylation by the AKT pathway promotes nuclear location.

DISEASE:
Note=Activation of telomerase has been implicated in cell immortalization and cancer cell pathogenesis.
Defects in TERT are associated with susceptibilty to aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
Note=Genetic variations in TERT are associated with coronary artery disease (CAD).
Defects in TERT are the cause of dyskeratosis congenital autosomal dominant type 2 (DKCA2) [MIM:613989]. A rare multisystem disorder caused by defective telomere maintenance. It is characterized by progressive bone marrow failure, and the clinical triad of reticulated skin hyperpigmentation, nail dystrophy, and mucosal leukoplakia. Common but variable features include premature graying, aplastic anemia, low platelets, osteoporosis, pulmonary fibrosis, and liver fibrosis among others. Early mortality is often associated with bone marrow failure, infections, fatal pulmonary complications, or malignancy.
Defects in TERT are the cause of dyskeratosis congenital autosomal recessive type 4 (DKCB4) [MIM:613989]. A rare multisystem disorder caused by defective telomere maintenance. It is characterized by progressive bone marrow failure, and the clinical triad of reticulated skin hyperpigmentation, nail dystrophy, and mucosal leukoplakia. Common but variable features include premature graying, aplastic anemia, low platelets, osteoporosis, pulmonary fibrosis, and liver fibrosis among others. Early mortality is often associated with bone marrow failure, infections, fatal pulmonary complications, or malignancy.
Defects in TERT are a cause of susceptibility to pulmonary fibrosis idiopathic (IPF) [MIM:178500]. Pulmonary fibrosis is a lung disease characterized by shortness of breath, radiographically evident diffuse pulmonary infiltrates, and varying degrees of inflammation and fibrosis on biopsy. It results in acute lung injury with subsequent scarring and endstage lung disease.

Similarity:
Belongs to the reverse transcriptase family. Telomerase subfamily.
Contains 1 reverse transcriptase domain.

SWISS:
O14746

Gene ID:
7015

Database links:

Entrez Gene: 7015 Human

Entrez Gene: 21752 Mouse

Entrez Gene: 301965 Rat

SwissProt: O14746 Human

SwissProt: O70372 Mouse

SwissProt: Q673L6 Rat




Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

端粒酶逆轉(zhuǎn)錄酶hTERT是構(gòu)成端粒酶的組分之一，是端粒酶活性的必需和限速成分，水平?jīng)Q定細(xì)胞端粒酶的活性。抑制hTERT可降低端粒酶的活性，從而抑制瘤細(xì)胞生長(zhǎng)。目前對(duì)hTERT的研究已成為端粒酶研究的熱點(diǎn)問(wèn)題，已發(fā)現(xiàn)TERT蛋白表達(dá)在腫瘤診斷中有重要意義，并制備了hTERT抗體及應(yīng)用核酶技術(shù)等來(lái)抑制hTERT蛋白的表達(dá)，抑制端粒酶活性，從而抑制腫瘤的生長(zhǎng)。
端粒反轉(zhuǎn)錄酶又稱端粒酶催化亞單位 （hTRT；Telomerase catalytic subunit；HEST2；Telomerase-associated protein 2；TP2；Telomerase reverse transcriptase；telomerase catalytic subunit）是細(xì)胞永生化及惡性腫瘤發(fā)生過(guò)程中的端粒酶活化的主要限速步驟。hTRT基因表達(dá)可以反映端粒酶活性，與端粒酶活性具有平行關(guān)系。