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磷酸化轉(zhuǎn)錄因子E2F-1抗體
  • 產(chǎn)品貨號：
    BN40226R
  • 中文名稱：
    磷酸化轉(zhuǎn)錄因子E2F-1抗體
  • 英文名稱：
    Rabbit anti-phospho-E2F1 (Ser337) Polyclonal antibody
  • 品牌：
    Biorigin

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  • BN40226R-100ul

    100ul

    ￥2470.00

    交叉反應(yīng)：Human(predicted:Mouse,Rat,Dog,Pig,Cow,Horse,GuineaPig) 推薦應(yīng)用：WB,ELISA

產(chǎn)品描述

英文名稱phospho-E2F1 (Ser337)
中文名稱磷酸化轉(zhuǎn)錄因子E2F-1抗體
別    名phospho-E2F1(Ser337); p-E2F1 (phospho-Ser337); E2F1 (phospho S337); E2F 1; E2F transcription factor 1; E2F-1; E2f1 E2F transcription factor 1; KIAA4009; mKIAA4009; OTTHUMP00000030661; PBR 3; PBR3; PRB binding protein E2F 1; PRB-binding protein E2F-1; RBAP 1; RBAP-1; RBAP1; RBBP 3; RBBP-3; RBBP3; RBP 3; RBP3; Retinoblastoma associated protein 1; Retinoblastoma binding protein 3; Retinoblastoma-associated protein 1; Retinoblastoma-binding protein 3; Transcription factor E2F1; E2F1_HUMAN.  
產(chǎn)品類型磷酸化抗體 
研究領(lǐng)域腫瘤  細(xì)胞生物  免疫學(xué)  轉(zhuǎn)錄調(diào)節(jié)因子  
抗體來源Rabbit
克隆類型Polyclonal
交叉反應(yīng)Human,  (predicted: Mouse, Rat, Dog, Pig, Cow, Horse, Guinea Pig, )
產(chǎn)品應(yīng)用WB=1:500-2000 ELISA=1:5000-10000 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量46kDa
細(xì)胞定位細(xì)胞核 
性    狀Liquid
濃    度1mg/ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human E2F1 around the phosphorylation site of Ser337:L(p-S)PL 
亞    型IgG
純化方法affinity purified by Protein A
儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
產(chǎn)品介紹E2F's are DNA binding proteins, which associate with negative regulators, such as the retinoblastoma p107 protein, resulting in an altered rate of gene transcription. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. E2F1 is proposed to be involved in several cellular processes that range from tumor suppressor, cell progression and oncogenesis. E2F1 overexpression can also drive cells into apoptosis.

Function:
Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis.

Subunit:
Component of the DRTF1/E2F transcription factor complex. Forms heterodimers with DP family members. The E2F-1 complex binds specifically hypophosphorylated retinoblastoma protein RB1. During the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex, rendering E2F transcriptionally active. Interacts with TRRAP, which probably mediates its interaction with histone acetyltransferase complexes, leading to transcription activation. Binds TOPBP1. Interacts with ARID3A (By similarity). Binds EAPP.

Subcellular Location:
Nucleus.

Post-translational modifications:
Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.

Similarity:
Belongs to the E2F/DP family.

SWISS:
Q01094

Gene ID:
1869

Database links:

Entrez Gene: 1869 Human

Entrez Gene: 13555 Mouse

Entrez Gene: 399489 Rat

Omim: 189971 Human

SwissProt: Q01094 Human

SwissProt: Q61501 Mouse

SwissProt: O09139 Rat

Unigene: 654393 Human

Unigene: 18036 Mouse

Unigene: 72471 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

E2F1—屬于調(diào)節(jié)性轉(zhuǎn)錄因子E2F家族。有學(xué)者認(rèn)為：E2F-1既可作為癌基因起作用，又可作為抑癌基因起作用。其不同可能由細(xì)胞中其他生長促進(jìn)或抑制性蛋白質(zhì)水平和（或）活性決定，同時與細(xì)胞所處環(huán)境及器官特異性有關(guān)。在控制細(xì)胞周期和腫瘤抑制基因蛋白的活性方面起關(guān)鍵作用。